منابع مشابه
Choice of Efficient Molecular Biology Techniques for Bacterial Cell Surface Display
For in vitro assessment of proteins, cell surface display is a novel technique. Using flow cytometry and / or iterative selection procedures like biopanning, libraries of heterologous polypeptides can de displayed and monitored on the surface of bacterial and yeast cells. However, this is not possible without the choice of efficient strains, reagents etc. and some high-throughput molecular biol...
متن کاملBacterial Cell Surface Deformation under External Loading
UNLABELLED Viscoelastic deformation of the contact volume between adhering bacteria and substratum surfaces plays a role in their adhesion and detachment. Currently, there are no deformation models that account for the heterogeneous structure and composition of bacteria, consisting of a relatively soft outer layer and a more rigid, hard core enveloped by a cross-linked peptidoglycan layer. The ...
متن کاملBacterial Cell Surface Heterogeneity: A Pathogen's Disguise
All interactions of microorganisms with their environment are surface phenomena, and therewith involve the properties of the microbial cell surface [1] and its possible disguise or hidden identity by an altered appearance. Since appearance is what one initially sees upon first encounter, a disguise always refers to surface properties, like cloths for people and hydrophobicity or charge for micr...
متن کاملVisualizing the bacterial cell surface: an overview.
The ultrastructure of bacteria is only accessible by electron microscopy. Our insights into the architecture of cells and cellular compartments such as the envelope and appendages is thus dependent on the progress of preparative and imaging techniques in electron microscopy. Here, I give a short overview of the development and characteristics of methods applied for imaging (components of) the b...
متن کاملBacterial cell surface damage due to centrifugal compaction.
Centrifugal damage has been known to alter bacterial cell surface properties and interior structures, including DNA. Very few studies exist on bacterial damage caused by centrifugation because of the difficulty in relating centrifugation speed and container geometry to the damage caused. Here, we provide a simple, versatile method of analysis for describing the compaction of bacteria during cen...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1989
ISSN: 0014-5793
DOI: 10.1016/0014-5793(89)80989-5